Hotspot

trnR(ACG)-rrn5

IRa · 145067-145316 · 250 bp

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Pi 0.008
MarkerSeek score 58.10
Primer available yes
Label trnR(ACG)-rrn5

Feature Metadata

label_name
trnR(ACG)-rrn5
feature_type
igs
region
IRa
length_bp
250
variable_sites
3
pi
0.008
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR(ACG)-rrn5_p1 CCACGTGCTCTAATCCTCTG GGTGACGATACTGTAGGGGA 55.0 55.0 57.8 57.9 333-334 bp 1.0 78.7
trnR(ACG)-rrn5_p2 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 368-369 bp 1.0 78.1
trnR(ACG)-rrn5_p3 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 371-372 bp 1.0 78.1
trnR(ACG)-rrn5_p4 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 369-370 bp 1.0 78.1
trnR(ACG)-rrn5_p5 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 372-373 bp 1.0 78.1