Hotspot

rps15-ycf1

SSC · 137139-138218 · 1080 bp

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Pi 0.0161

MarkerSeek score 70.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 1080
variable_sites: 24
pi: 0.0161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCGACAGACCCCTTGTTTTC	AGCTTGTATGAATCGGTATTGGT	50.0	39.1	57.8	58.0	1269-1347 bp	1.0	59.2
rps15-ycf1_p2	CGACAGACCCCTTGTTTTCT	AGCTTGTATGAATCGGTATTGGT	50.0	39.1	57.7	58.0	1268-1346 bp	1.0	59.1
rps15-ycf1_p3	TCGACAGACCCCTTGTTTTC	TGGTAGTCGTTTCAGTATGTCA	50.0	40.9	57.8	56.9	1233-1311 bp	1.0	58.5
rps15-ycf1_p4	CGACAGACCCCTTGTTTTCT	TGGTAGTCGTTTCAGTATGTCA	50.0	40.9	57.7	56.9	1232-1310 bp	1.0	58.5
rps15-ycf1_p5	TCGACAGACCCCTTGTTTTC	GCTTGTATGAATCGGTATTGGT	50.0	40.9	57.8	56.6	1268-1346 bp	1.0	56.1