Hotspot

ndhE-ndhG

SSC · 131015-131387 · 373 bp

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Pi 0.00894

MarkerSeek score 62.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 373
variable_sites: 5
pi: 0.00894
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GTGCTCTAACCATGCTTCGA	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	495-506 bp	1.0	78.0
ndhE-ndhG_p2	TGCTCTAACCATGCTTCGAC	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	494-505 bp	1.0	78.0
ndhE-ndhG_p3	AGTGCTCTAACCATGCTTCG	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	496-507 bp	1.0	77.9
ndhE-ndhG_p4	GCTCTAACCATGCTTCGACT	AGTTGCCTTGATAGGTGCAA	50.0	45.0	57.7	57.4	493-504 bp	1.0	75.6
ndhE-ndhG_p5	TGCTCTAACCATGCTTCGAC	AGTTGCCTTGATAGGTGCAAT	50.0	42.9	58.0	57.9	494-505 bp	1.0	73.9