Hotspot

trnL(CAA)-ndhB

IRb · 104672-105758 · 1087 bp

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Pi 0.048

MarkerSeek score 86.90

Primer available yes

Label trnL(CAA)-ndhB

Feature Metadata

label_name: trnL(CAA)-ndhB
feature_type: igs
region: IRb
length_bp: 1087
variable_sites: 73
pi: 0.048
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(CAA)-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	1147-1323 bp	1.0	87.0
trnL(CAA)-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	1145-1321 bp	1.0	87.0
trnL(CAA)-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	1142-1318 bp	1.0	85.5
trnL(CAA)-ndhB_p4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	1146-1322 bp	1.0	84.9
trnL(CAA)-ndhB_p5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.3	57.9	1144-1320 bp	1.0	84.7