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rpl16-rps3

LSC · 92825-92980 · 156 bp

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Pi 0.0171

MarkerSeek score 62.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 4
pi: 0.0171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AACGAGTCACACACTAAGCA	TTCCCCTACAAACCATTCGC	45.0	50.0	57.1	58.2	284 bp	1.0	72.7
rpl16-rps3_p2	AACGAGTCACACACTAAGCA	CCTACAAACCATTCGCGCTA	45.0	50.0	57.1	58.4	280 bp	1.0	71.3
rpl16-rps3_p3	ACGAGTCACACACTAAGCAT	TTCCCCTACAAACCATTCGC	45.0	50.0	56.9	58.2	283 bp	1.0	70.9
rpl16-rps3_p4	CAACGAGTCACACACTAAGC	TTCCCCTACAAACCATTCGC	50.0	50.0	56.8	58.2	285 bp	1.0	70.1
rpl16-rps3_p5	ACGAGTCACACACTAAGCAT	CCTACAAACCATTCGCGCTA	45.0	50.0	56.9	58.4	279 bp	1.0	69.5