Hotspot

accD-psaI

LSC · 66465-68602 · 2138 bp

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Pi 0.0124

MarkerSeek score 55.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 2138
variable_sites: 37
pi: 0.0124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCACAAGCGGCTGAGTATTC	ATGATCCCCCTTTGACCAGA	50.0	50.0	58.0	58.0	2501-2574 bp	1.0	85.1
accD-psaI_p2	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2255-2328 bp	1.0	85.0
accD-psaI_p3	TCACAAGCGGCTGAGTATTC	TTCGCAAGGAGCCACATATC	50.0	50.0	58.0	58.0	2406-2479 bp	1.0	84.8
accD-psaI_p4	TCACAAGCGGCTGAGTATTC	TTGTTTTTCGAGACCCCGTT	50.0	45.0	58.0	58.0	2721-2794 bp	1.0	84.6
accD-psaI_p5	GCGAGAAAATCACCCGTTTG	ATGATCCCCCTTTGACCAGA	50.0	50.0	58.0	58.0	2821-2894 bp	1.0	84.4