Hotspot

trnT(UGU)-trnL(UAA)

LSC · 52663-53889 · 1227 bp

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Pi 0.00874
MarkerSeek score 62.50
Primer available yes
Label trnT(UGU)-trnL(UAA)

Feature Metadata

label_name
trnT(UGU)-trnL(UAA)
feature_type
igs
region
LSC
length_bp
1227
variable_sites
16
pi
0.00874
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(UGU)-trnL(UAA)_p1 GGAAAAAGCCAGCTATCGGA ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1326-1375 bp 1.0 82.3
trnT(UGU)-trnL(UAA)_p2 AGGGAAAAAGCCAGCTATCG ACCAATTTCGCCATATCCCC 50.0 50.0 58.0 58.0 1328-1377 bp 1.0 82.3
trnT(UGU)-trnL(UAA)_p3 GGAAAAAGCCAGCTATCGGA TCCATACCAAGGCTCAATGC 50.0 50.0 58.0 57.9 1365-1414 bp 1.0 81.8
trnT(UGU)-trnL(UAA)_p4 AGGGAAAAAGCCAGCTATCG TCCATACCAAGGCTCAATGC 50.0 50.0 58.0 57.9 1367-1416 bp 1.0 81.8
trnT(UGU)-trnL(UAA)_p5 GGAAAAAGCCAGCTATCGGA TAGCGTCTACCAATTTCGCC 50.0 50.0 58.0 58.1 1334-1383 bp 1.0 81.8