Hotspot

psaA-ycf3

LSC · 46761-47447 · 687 bp

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Pi 0.00388

MarkerSeek score 66.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 4
pi: 0.00388
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TGGTTGAAGATAACGAGGCG	50.0	50.0	58.1	58.0	727-802 bp	1.0	83.8
psaA-ycf3_p2	TGGCCCATTCCTCAAAAGAC	TGGTTGAAGATAACGAGGCG	50.0	50.0	58.1	58.0	724-799 bp	1.0	83.8
psaA-ycf3_p3	GTCTGGCCCATTCCTCAAAA	GTTGAAGATAACGAGGCGGT	50.0	50.0	58.1	58.0	725-800 bp	1.0	83.8
psaA-ycf3_p4	TGGCCCATTCCTCAAAAGAC	GTTGAAGATAACGAGGCGGT	50.0	50.0	58.1	58.0	722-797 bp	1.0	83.8
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	TGGTTGAAGATAACGAGGCG	50.0	50.0	57.8	58.0	725-800 bp	1.0	82.6