Hotspot

ycf1

SSC · 122321-127807 · 5487 bp

Back to Antirrhinum

Pi 0.0034

MarkerSeek score 40.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5487
variable_sites: 36
pi: 0.0034
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCATTCCTTGTCTTTCAAAAGA	TCAATTTCGTTTCTTTCAACT	34.8	28.6	56.5	52.2	233 bp	1.0	41.4
ycf1_p2	ACCATTCCTTGTCTTTCAAAAGA	TTCAATTTCGTTTCTTTCAACT	34.8	27.3	56.5	53.1	234 bp	1.0	41.4
ycf1_p3	AACCATTCCTTGTCTTTCAAAAGA	TCAATTTCGTTTCTTTCAACT	33.3	28.6	57.2	52.2	234 bp	1.0	41.4
ycf1_p4	ACCATTCCTTGTCTTTCAAAAGAA	TCAATTTCGTTTCTTTCAACT	33.3	28.6	57.2	52.2	233 bp	1.0	41.4
ycf1_p5	ACCATTCCTTGTCTTTCAAAAGAA	TTCAATTTCGTTTCTTTCAACT	33.3	27.3	57.2	53.1	234 bp	1.0	41.4