Hotspot

ndhD

SSC · 114043-115572 · 1530 bp

Back to Antirrhinum

Pi 0.00163

MarkerSeek score 36.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 5
pi: 0.00163
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGAAGGGTCCAAATTCAGCA	GATACCCTGGGACGGATGTA	50.0	55.0	58.1	58.0	2153 bp	1.0	80.1
ndhD_p2	TTGGAGTTGGGACCCAAAAG	GATACCCTGGGACGGATGTA	50.0	55.0	57.9	58.0	2239 bp	1.0	80.0
ndhD_p3	CTAACGAAGCATGGGGATCC	GATACCCTGGGACGGATGTA	55.0	55.0	58.1	58.0	2261 bp	1.0	79.7
ndhD_p4	TTGGTGTGAACTGGCAAGAA	GATACCCTGGGACGGATGTA	45.0	55.0	57.9	58.0	1970 bp	1.0	79.7
ndhD_p5	AACTTGGTGTGAACTGGCAA	GATACCCTGGGACGGATGTA	45.0	55.0	58.2	58.0	1973 bp	1.0	79.5