Hotspot

rpl32-trnL-UAG

SSC · 112006-112654 · 649 bp

Back to Antirrhinum

Pi 0.00752
MarkerSeek score 60.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
649
variable_sites
9
pi
0.00752
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAAGCCTTTGCCTTGGGTA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 771-779 bp 1.0 80.0
rpl32-trnL-UAG_p2 AAAGCCTTTGCCTTGGGTAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 770-778 bp 1.0 80.0
rpl32-trnL-UAG_p3 AAGCCTTTGCCTTGGGTAAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 769-777 bp 1.0 80.0
rpl32-trnL-UAG_p4 TAAAAGCCTTTGCCTTGGGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 772-780 bp 1.0 80.0
rpl32-trnL-UAG_p5 GCGTTAAAAGCCTTTGCCTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 776-784 bp 1.0 79.6