Hotspot

ndhF-rpl32

SSC · 111009-111828 · 820 bp

Back to Antirrhinum

Pi 0.00732

MarkerSeek score 60.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 820
variable_sites: 11
pi: 0.00732
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACGAAAGGTATGATCCACGC	ACCCAAGGCAAAGGCTTTTA	50.0	45.0	58.1	57.9	966-971 bp	1.0	79.7
ndhF-rpl32_p2	ACGAAAGGTATGATCCACGC	TACCCAAGGCAAAGGCTTTT	50.0	45.0	58.1	57.9	967-972 bp	1.0	79.7
ndhF-rpl32_p3	ACGAAAGGTATGATCCACGC	TTACCCAAGGCAAAGGCTTT	50.0	45.0	58.1	57.9	968-973 bp	1.0	79.7
ndhF-rpl32_p4	CGAAAGGTATGATCCACGCA	ACCCAAGGCAAAGGCTTTTA	50.0	45.0	58.1	57.9	965-970 bp	1.0	79.7
ndhF-rpl32_p5	CGAAAGGTATGATCCACGCA	TACCCAAGGCAAAGGCTTTT	50.0	45.0	58.1	57.9	966-971 bp	1.0	79.7