Hotspot

petA-psbJ

LSC · 62230-63266 · 1037 bp

Back to Antirrhinum

Pi 0.00241

MarkerSeek score 54.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 5
pi: 0.00241
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.0	57.5	1144-1146 bp	1.0	76.6
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.0	57.5	1178-1180 bp	1.0	76.3
petA-psbJ_p3	GGCATCTGTGATTTTGGCAC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.0	57.4	1143-1145 bp	1.0	76.1
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	CCGGATTAGGTTCATCCCTG	50.0	55.0	58.0	57.4	1177-1179 bp	1.0	75.8
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	TCCGGATTAGGTTCATCCCT	50.0	50.0	57.8	57.5	1161-1163 bp	1.0	75.3