Hotspot

ycf4-cemA

LSC · 59625-60362 · 738 bp

Back to Antirrhinum

Pi 0.00632

MarkerSeek score 64.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 9
pi: 0.00632
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	862-889 bp	1.0	79.1
ycf4-cemA_p2	TATTTCTTGCGCGTCCCAAT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.3	58.1	838-865 bp	1.0	78.9
ycf4-cemA_p3	CGCGTCCCAATTGAAGTTTT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.6	58.1	829-856 bp	1.0	77.6
ycf4-cemA_p4	AACAAAAAGCCGCGGAATTG	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.5	58.1	861-888 bp	1.0	76.8
ycf4-cemA_p5	AATTGAACAAAAAGCCGCGG	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.5	58.1	866-893 bp	1.0	76.8