Hotspot

trnG-GCC-trnfM-CAU

LSC · 35262-35412 · 151 bp

Back to Antirrhinum

Pi 0.0232
MarkerSeek score 59.60
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
151
variable_sites
6
pi
0.0232
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 217 bp 1.0 81.4
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 218 bp 1.0 81.4
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 253 bp 1.0 81.2
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 254 bp 1.0 81.2
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC GTCACGGGTTCAAATCCTGT 50.0 50.0 58.1 58.1 211 bp 1.0 80.9