Hotspot

psbZ-trnG-GCC

LSC · 34954-35190 · 237 bp

Back to Antirrhinum

Pi 0.00422
MarkerSeek score 32.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
237
variable_sites
2
pi
0.00422
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 405 bp 1.0 78.3
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 406 bp 1.0 78.3
psbZ-trnG-GCC_p3 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 403 bp 1.0 77.9
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC GAATCGAACCCGCATCTTCT 55.0 50.0 58.3 58.1 404 bp 1.0 77.9
psbZ-trnG-GCC_p5 CCGTCGTATTTGCTTCTCCT AATCGAACCCGCATCTTCTC 50.0 50.0 57.7 58.1 414 bp 1.0 77.6