Hotspot

rpoB

LSC · 35208-38420 · 3213 bp

Back to Anthyllis

Pi 0.00228

MarkerSeek score 56.70

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 11
pi: 0.00228
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GGCGGAGATCCAGTATTTCC	TCATCAAGTGTACCCATTCGA	55.0	42.9	57.8	56.7	215 bp	1.0	61.7
rpoB_p2	GGGCGGAGATCCAGTATTTC	TCATCAAGTGTACCCATTCGA	55.0	42.9	57.8	56.7	216 bp	1.0	61.7
rpoB_p3	GGCGGAGATCCAGTATTTCC	GGCCAATCCAAATTGATCTTGT	55.0	40.9	57.8	57.5	285 bp	1.0	59.7
rpoB_p4	GGGCGGAGATCCAGTATTTC	GGCCAATCCAAATTGATCTTGT	55.0	40.9	57.8	57.5	286 bp	1.0	59.7
rpoB_p5	GGCGGAGATCCAGTATTTCC	TCATCAAGTGTACCCATTCGAA	55.0	40.9	57.8	57.5	215 bp	1.0	59.4