Hotspot

psbC

LSC · 26259-27680 · 1422 bp

Back to Anthyllis

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 3
pi: 0.00141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	ATGATCTGCTCGACTCAACC	GAAGGTATTCGTGCGTGGAT	50.0	50.0	57.4	58.1	1542 bp	1.0	74.9
psbC_p2	CTGCTCGACTCAACCCAATA	GAAGGTATTCGTGCGTGGAT	50.0	50.0	57.3	58.1	1537 bp	1.0	74.3
psbC_p3	ATGATCTGCTCGACTCAACC	GCAGCGGAAGATCCTGAATT	50.0	50.0	57.4	58.3	1596 bp	1.0	72.8
psbC_p4	ATGATCTGCTCGACTCAACC	AAGGTATTCGTGCGTGGATG	50.0	50.0	57.4	58.3	1541 bp	1.0	72.6
psbC_p5	ATGATCTGCTCGACTCAACC	GGATGGCAGCTCAAGATCAG	50.0	55.0	57.4	58.4	1526 bp	1.0	72.2