Hotspot

accD

LSC · 57793-59355 · 1563 bp

Back to Anthyllis

Pi 0.00469

MarkerSeek score 49.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1563
variable_sites: 11
pi: 0.00469
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGGGATTTTTCATCGAATGACT	ATTGAGTATGTAGGGTGAGT	36.4	40.0	56.1	52.7	1665-1683 bp	1.0	40.5
accD_p2	TTGGGATTTTTCATCGAATGACT	ATTGAGTATGTAGGGTGAGT	34.8	40.0	56.8	52.7	1666-1684 bp	1.0	40.5
accD_p3	TGGGATTTTTCATCGAATGAC	ATTGAGTATGTAGGGTGAGT	38.1	40.0	54.5	52.7	1665-1683 bp	1.0	40.5
accD_p4	TTTGGGATTTTTCATCGAATGACT	ATTGAGTATGTAGGGTGAGT	33.3	40.0	57.4	52.7	1667-1685 bp	1.0	40.5
accD_p5	TGGGATTTTTCATCGAATGACT	TTGAGTATGTAGGGTGAGTA	36.4	40.0	56.1	52.1	1664-1682 bp	1.0	40.5