Hotspot

trnP-UGG-psaJ

LSC · 65444-66098 · 655 bp

Back to Anthyllis

Pi 0.00102

MarkerSeek score 49.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	752-765 bp	1.0	79.1
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	751-764 bp	1.0	79.1
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	763-776 bp	1.0	79.0
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	762-775 bp	1.0	79.0
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GTACTTACCACTGGTGCCAC	50.0	55.0	58.1	58.2	765-778 bp	1.0	79.0