Hotspot

cemA-petA

LSC · 61489-61747 · 259 bp

Back to Anthyllis

Pi 0.00515
MarkerSeek score 29.60
Primer available yes
Label cemA-petA

Feature Metadata

label_name
cemA-petA
feature_type
igs
region
LSC
length_bp
259
variable_sites
2
pi
0.00515
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
29.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
cemA-petA_p1 AACACGTCTTCAAACCCTCC CGTGGGACGATAGCTTTGAA 50.0 50.0 58.0 58.0 1145 bp 1.0 80.0
cemA-petA_p2 AACACGTCTTCAAACCCTCC GGGCAAAAATCGGATATGCG 50.0 50.0 58.0 57.9 837 bp 1.0 79.7
cemA-petA_p3 GTCTTCAAACCCTCCGTACC CGTGGGACGATAGCTTTGAA 55.0 50.0 57.9 58.0 1140 bp 1.0 79.6
cemA-petA_p4 AACACGTCTTCAAACCCTCC GCCAATTCAAAACCTTCCGG 50.0 50.0 58.0 57.9 1072 bp 1.0 79.4
cemA-petA_p5 AACACGTCTTCAAACCCTCC GGCCAATTCAAAACCTTCCG 50.0 50.0 58.0 57.9 1073 bp 1.0 79.4