Hotspot

trnC-GCA-rpoB

LSC · 34281-35207 · 927 bp

Back to Anthyllis

Pi 0.00144

MarkerSeek score 59.90

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 927
variable_sites: 2
pi: 0.00144
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	GGATTTGATTCAACCCCGGT	50.0	50.0	58.1	58.2	1043-1044 bp	1.0	78.4
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	TGGATTTGATTCAACCCCGG	50.0	50.0	58.1	58.2	1044-1045 bp	1.0	78.4
trnC-GCA-rpoB_p3	TCTTTCTTGCAGATCCAGGC	GGATTTGATTCAACCCCGGT	50.0	50.0	57.9	58.2	1068-1069 bp	1.0	78.1
trnC-GCA-rpoB_p4	TCTTTCTTGCAGATCCAGGC	TGGATTTGATTCAACCCCGG	50.0	50.0	57.9	58.2	1069-1070 bp	1.0	78.1
trnC-GCA-rpoB_p5	CCGGATTTGAACTGGGGAAA	ACCCCGGTAGTGTAGACATT	50.0	50.0	58.1	57.8	1031-1032 bp	1.0	77.7