Hotspot

psbD-trnT-GGU

LSC · 28690-29907 · 1218 bp

Back to Anthyllis

Pi 0.00109

MarkerSeek score 59.40

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 2
pi: 0.00109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	GAAACGGTCCCTACGTAACC	GTGGTAGAGTAACGCCATGG	55.0	55.0	58.0	58.1	1360-1368 bp	1.0	79.6
psbD-trnT-GGU_p2	GAAACGGTCCCTACGTAACC	TCAGTGGTAGAGTAACGCCA	55.0	50.0	58.0	58.1	1363-1371 bp	1.0	79.4
psbD-trnT-GGU_p3	CACAAAAACGAAACGGTCCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1369-1377 bp	1.0	78.2
psbD-trnT-GGU_p4	CCACAAAAACGAAACGGTCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1370-1378 bp	1.0	78.2
psbD-trnT-GGU_p5	CCCACAAAAACGAAACGGTC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1371-1379 bp	1.0	78.2