Hotspot

trnK-UUU-rbcL

LSC · 4473-5237 · 765 bp

Back to Anthyllis

Pi 0.00784

MarkerSeek score 64.80

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 8
pi: 0.00784
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AAAGCCGAGTACTCTACCGT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	869-875 bp	1.0	79.8
trnK-UUU-rbcL_p2	AAGCCGAGTACTCTACCGTT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	868-874 bp	1.0	79.8
trnK-UUU-rbcL_p3	AAGCCGAGTACTCTACCGTT	AATTATCCGTGAAGCGTGCA	50.0	45.0	58.2	58.3	893-899 bp	1.0	77.9
trnK-UUU-rbcL_p4	AAGCCGAGTACTCTACCGTT	ATTATCCGTGAAGCGTGCAA	50.0	45.0	58.2	58.3	892-898 bp	1.0	77.9
trnK-UUU-rbcL_p5	AAGCCGAGTACTCTACCGTT	TATCCGTGAAGCGTGCAAAT	50.0	45.0	58.2	58.3	890-896 bp	1.0	77.9