Hotspot

trnH-GUG-psbA

LSC · 131-404 · 274 bp

Back to Anthyllis

Pi 0.00973
MarkerSeek score 34.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
274
variable_sites
4
pi
0.00973
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG ATCCGATTGGTCAAGGAAGC 55.0 50.0 58.1 58.0 872-900 bp 1.0 79.5
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA ATCCGATTGGTCAAGGAAGC 50.0 50.0 57.9 58.0 886-914 bp 1.0 79.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC ATCCGATTGGTCAAGGAAGC 50.0 50.0 57.9 58.0 885-913 bp 1.0 79.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC ATCCGATTGGTCAAGGAAGC 50.0 50.0 57.9 58.0 887-915 bp 1.0 79.5
trnH-GUG-psbA_p5 TTTATATGGGCGAACGACGG ATCCGATTGGTCAAGGAAGC 50.0 50.0 58.1 58.0 933-961 bp 1.0 79.3