Hotspot

ndhI

SSC · 126007-126549 · 543 bp

Back to Anthurium

Pi 0.47

MarkerSeek score 80.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 543
variable_sites: 225
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TCTTGTATATGCTACGTCCCT	ACCAGAAGCAGAGGAGGAAT	42.9	50.0	55.7	58.0	1650 bp	0.5	33.2
ndhI_p2	TCTTGTATATGCTACGTCCCT	TTGGGGGTGGAATTTATGGC	42.9	50.0	55.7	58.1	1740 bp	0.5	32.5
ndhI_p3	TCTTGTATATGCTACGTCCCT	TGGTTTTTGGGGGTGGAATT	42.9	45.0	55.7	57.8	1746 bp	0.5	31.9
ndhI_p4	TCTTGTATATGCTACGTCCCT	GGGTGGAATTTATGGCGTCA	42.9	50.0	55.7	58.2	1736 bp	0.5	31.5
ndhI_p5	TTCTTGTATATGCTACGTCCCT	ACCAGAAGCAGAGGAGGAAT	40.9	50.0	56.4	58.0	1651 bp	0.5	31.3