Hotspot

ndhD

SSC · 120636-122135 · 1500 bp

Back to Anthurium

Pi 0.473

MarkerSeek score 82.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 634
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTCTCTGGAGCAGTATGGG	GAACCGAAGATTGCGTAGGT	55.0	50.0	57.9	58.0	2146 bp	0.5	59.3
ndhD_p2	TCTCTCTGGAGCAGTATGGG	TATGGCATGAGACAACTCGC	55.0	50.0	57.9	58.1	2071 bp	0.5	58.9
ndhD_p3	AATGAGGCATGGGGATCCTA	GAACCGAAGATTGCGTAGGT	50.0	50.0	57.9	58.0	2124 bp	0.5	58.8
ndhD_p4	TAATGAGGCATGGGGATCCT	GAACCGAAGATTGCGTAGGT	50.0	50.0	57.9	58.0	2125 bp	0.5	58.8
ndhD_p5	TGAGGCATGGGGATCCTATT	GAACCGAAGATTGCGTAGGT	50.0	50.0	57.9	58.0	2122 bp	0.5	58.8