Hotspot

ccsA

SSC · 119515-120486 · 972 bp

Back to Anthurium

Pi 0.451

MarkerSeek score 80.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 367
pi: 0.451
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.0	58.1	1619 bp	0.5	59.4
ccsA_p2	TGAAATCGGTAGACACGCTG	TTGCATTGCCAGGAATGAGT	50.0	45.0	58.0	58.1	1618 bp	0.5	59.3
ccsA_p3	GTGAAATCGGTAGACACGCT	CATTGCCAGGAATGAGTGGT	50.0	50.0	58.0	58.2	1616 bp	0.5	58.7
ccsA_p4	TGAAATCGGTAGACACGCTG	CATTGCCAGGAATGAGTGGT	50.0	50.0	58.0	58.2	1615 bp	0.5	58.7
ccsA_p5	GTGAAATCGGTAGACACGCT	CTTCTCTTGCATTGCCAGGA	50.0	50.0	58.0	58.2	1625 bp	0.5	58.6