Hotspot

ndhH-rps15

SSC · 130009-130112 · 104 bp

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Pi 0.466

MarkerSeek score 74.40

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 104
variable_sites: 41
pi: 0.466
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	TCCGGAAGCTCGTAACATTG	AGTGCGTGAGTGAGACATTC	50.0	50.0	58.0	57.9	1465 bp	0.5	59.4
ndhH-rps15_p2	CATTGGTCCTGATAAGCCCC	AGTGCGTGAGTGAGACATTC	55.0	50.0	58.0	57.9	1450 bp	0.5	59.3
ndhH-rps15_p3	CCAATAATGCCCACTCCCTC	AGTGCGTGAGTGAGACATTC	55.0	50.0	58.0	57.9	1410 bp	0.5	59.3
ndhH-rps15_p4	TCCGGAAGCTCGTAACATTG	GCGTGAGTGAGACATTCGAT	50.0	50.0	58.0	58.1	1462 bp	0.5	59.3
ndhH-rps15_p5	TGCTTCCTCTCCCCCAATAA	AGTGCGTGAGTGAGACATTC	50.0	50.0	58.0	57.9	1423 bp	0.5	59.3