Hotspot

ndhF-rpl32

SSC · 117235-118474 · 1240 bp

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Pi 0.434

MarkerSeek score 82.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1240
variable_sites: 486
pi: 0.434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGCAATTGGTCGTGTGAAC	TACTCGTTTGGTCGCACAAA	50.0	45.0	58.0	58.1	1912 bp	0.5	59.2
ndhF-rpl32_p2	CTGCAATTGGTCGTGTGAAC	TTACTCGTTTGGTCGCACAA	50.0	45.0	58.0	58.1	1913 bp	0.5	59.2
ndhF-rpl32_p3	AATAGGGAGGCACACAGAGT	TACTCGTTTGGTCGCACAAA	50.0	45.0	58.0	58.1	2096 bp	0.5	59.2
ndhF-rpl32_p4	AATAGGGAGGCACACAGAGT	TTACTCGTTTGGTCGCACAA	50.0	45.0	58.0	58.1	2097 bp	0.5	59.2
ndhF-rpl32_p5	AGGAAAAGCCCACATACGAC	TACTCGTTTGGTCGCACAAA	50.0	45.0	57.9	58.1	1527 bp	0.5	58.6