Hotspot

trnN-GUU-ndhF

SSC · 113908-114996 · 1089 bp

Back to Anthurium

Pi 0.289

MarkerSeek score 82.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 1089
variable_sites: 288
pi: 0.289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	CCTGGCCCAAATCAGCATAT	AAAGGTCGTAACTGGGGGTA	50.0	50.0	58.0	58.0	1817 bp	0.5	59.7
trnN-GUU-ndhF_p2	ACCTGGGGCTTTTTACAAGG	AAAGGTCGTAACTGGGGGTA	50.0	50.0	58.0	58.0	1585 bp	0.5	59.7
trnN-GUU-ndhF_p3	CGGGAGAATTGATGACTGCA	AAAGGTCGTAACTGGGGGTA	50.0	50.0	58.0	58.0	1663 bp	0.5	59.5
trnN-GUU-ndhF_p4	AACGGTAAAAACGAGCCCTT	AAAGGTCGTAACTGGGGGTA	45.0	50.0	58.0	58.0	1447 bp	0.5	59.5
trnN-GUU-ndhF_p5	ACGGTAAAAACGAGCCCTTT	AAAGGTCGTAACTGGGGGTA	45.0	50.0	58.0	58.0	1446 bp	0.5	59.5