Hotspot

ycf1

SSC · 124808-130189 · 5382 bp

Back to Anthriscus

Pi 0.0216

MarkerSeek score 52.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5382
variable_sites: 173
pi: 0.0216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACAAACGGATGGGATCGAT	TGAGGACTGGACCAAAATGG	50.0	50.0	57.8	57.4	747-756 bp	1.0	77.5
ycf1_p2	GGCCTGTGGGTTCTTTTTCT	TGAGGACTGGACCAAAATGG	50.0	50.0	58.3	57.4	410-419 bp	1.0	76.4
ycf1_p3	GACAAACGGATGGGATCGAT	TCAAGGTGATATGACCCCCA	50.0	50.0	57.8	57.7	1161 bp	0.333333	49.2
ycf1_p4	GGCCTGTGGGTTCTTTTTCT	TCAAGGTGATATGACCCCCA	50.0	50.0	58.3	57.7	818 bp	0.333333	48.7
ycf1_p5	ACAGACAAACGGATGGGATC	TCAAGGTGATATGACCCCCA	50.0	50.0	57.6	57.7	1164 bp	0.333333	47.9