Hotspot

rpl16

LSC · 81932-83246 · 1315 bp

Back to Anthriscus

Pi 0.024

MarkerSeek score 60.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1315
variable_sites: 46
pi: 0.024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CAAAAGGGTCTGGGGTTGAA	GGGTTCCCCTTCAAACCATT	50.0	50.0	57.9	58.0	1732-1768 bp	1.0	82.9
rpl16_p2	TTCAAAAGGGTCTGGGGTTG	GGGTTCCCCTTCAAACCATT	50.0	50.0	57.9	58.0	1734-1770 bp	1.0	82.9
rpl16_p3	CAAAAGGGTCTGGGGTTGAA	GTCGAGGGAATTGCACGTAT	50.0	50.0	57.9	58.1	2111-2147 bp	1.0	82.3
rpl16_p4	ACACATCAATTCTCGAGCCC	GGGTTCCCCTTCAAACCATT	50.0	50.0	58.0	58.0	1771 bp	0.333333	52.9
rpl16_p5	ACACATCAATTCTCGAGCCC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2150 bp	0.333333	52.6