Hotspot

rpl32-trnL-UAG

SSC · 114326-115173 · 848 bp

Back to Anthriscus

Pi 0.0349
MarkerSeek score 74.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
848
variable_sites
43
pi
0.0349
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAGCCTTAAAAGCGTTGTC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 952-983 bp 1.0 83.1
rpl32-trnL-UAG_p2 GCAGCCTTAAAAGCGTTGTC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 953-984 bp 1.0 83.1
rpl32-trnL-UAG_p3 GCAGCCTTAAAAGCGTTGTC CTCGCACTGCTTCCTAAGAG 50.0 55.0 58.3 58.1 973-1004 bp 1.0 82.4
rpl32-trnL-UAG_p4 GCAGCCTTAAAAGCGTTGTC CTCTCGCACTGCTTCCTAAG 50.0 55.0 58.3 58.1 975-1006 bp 1.0 82.4
rpl32-trnL-UAG_p5 GCAGCCTTAAAAGCGTTGTC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.3 57.9 962-993 bp 1.0 82.2