Hotspot

ndhF-rpl32

SSC · 113214-114160 · 947 bp

Back to Anthriscus

Pi 0.0357

MarkerSeek score 72.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 947
variable_sites: 50
pi: 0.0357
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCCACGCATATTGATATGTCTGT	TCCAAATATTTTTACGAATACGCT	39.1	29.2	57.8	55.3	1036-1099 bp	1.0	44.9
ndhF-rpl32_p2	TCCACGCATATTGATATGTCTGT	TCCCTTTCCAAATATTTTTACGA	39.1	30.4	57.8	54.3	1042-1105 bp	1.0	44.9
ndhF-rpl32_p3	GGAAGTGGAAGGAAAGGAAT	TCCAAATATTTTTACGAATACGCT	45.0	29.2	54.9	55.3	1058-1121 bp	1.0	44.8
ndhF-rpl32_p4	GGAAGTGGAAGGAAAGGAAT	TCCCTTTCCAAATATTTTTACGA	45.0	30.4	54.9	54.3	1064-1127 bp	1.0	44.8
ndhF-rpl32_p5	GGAAGTGGAAGGAAAGGAATT	TCCAAATATTTTTACGAATACGCT	42.9	29.2	55.7	55.3	1058-1121 bp	1.0	44.8