Hotspot

rpl16-rps3

LSC · 83247-83394 · 148 bp

Back to Anthriscus

Pi 0.0616

MarkerSeek score 76.40

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 13
pi: 0.0616
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAAAACCAACGAGTCGCACA	GGGTTCCCCTTCAAACCATT	45.0	50.0	58.9	58.0	284-286 bp	1.0	76.7
rpl16-rps3_p2	CCAACGAGTCGCACACTAAG	GGGTTCCCCTTCAAACCATT	55.0	50.0	58.9	58.0	279-281 bp	1.0	76.6
rpl16-rps3_p3	CGAGTCGCACACTAAGCAT	GGGTTCCCCTTCAAACCATT	52.6	50.0	58.0	58.0	275-277 bp	1.0	75.9
rpl16-rps3_p4	AAAAACCAACGAGTCGCACA	CCCTTCAAACCATTCGAGCT	45.0	50.0	58.9	58.2	278-280 bp	1.0	75.6
rpl16-rps3_p5	CCAACGAGTCGCACACTAAG	CCCTTCAAACCATTCGAGCT	55.0	50.0	58.9	58.2	273-275 bp	1.0	75.5