Hotspot

ndhC-trnV-UAC

LSC · 51334-52414 · 1081 bp

Back to Anthriscus

Pi 0.0322

MarkerSeek score 71.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1081
variable_sites: 50
pi: 0.0322
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	1084-1125 bp	1.0	47.0
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	31.8	55.0	53.9	58.1	1099-1140 bp	1.0	46.9
ndhC-trnV-UAC_p3	ATCATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	1100-1141 bp	1.0	46.9
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	30.4	55.0	54.5	58.1	1099-1140 bp	1.0	46.9
ndhC-trnV-UAC_p5	CATATTCGTAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	33.3	55.0	52.3	58.1	1098-1139 bp	1.0	46.9