Hotspot

trnS-GCU-trnG-GCC

LSC · 8441-8979 · 539 bp

Back to Anthriscus

Pi 0.0352

MarkerSeek score 75.10

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 539
variable_sites: 28
pi: 0.0352
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	TGAAGGGGATTACTCGAACG	45.0	50.0	58.0	57.0	625-630 bp	1.0	76.8
trnS-GCU-trnG-GCC_p2	ACGGAAAGAGAGGGATTCGA	TGAAGGGGATTACTCGAACG	50.0	50.0	58.2	57.0	674-679 bp	1.0	75.5
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	TTGAAGGGGATTACTCGAACG	45.0	47.6	58.0	57.8	626-631 bp	1.0	74.5
trnS-GCU-trnG-GCC_p4	ATTAGCAATCCGCCGCTTTA	GAAGGGGATTACTCGAACGA	45.0	50.0	58.0	56.8	624-629 bp	1.0	74.5
trnS-GCU-trnG-GCC_p5	GCTTTAGTCCACTCAGCCAT	TGAAGGGGATTACTCGAACG	50.0	50.0	57.6	57.0	611-616 bp	1.0	73.9