Hotspot

trnH-GUG-psbA

LSC · 78-257 · 180 bp

Back to Anthriscus

Pi 0.0599
MarkerSeek score 76.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
180
variable_sites
16
pi
0.0599
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 247-251 bp 1.0 84.9
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 261-265 bp 1.0 84.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 260-264 bp 1.0 84.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.9 57.9 262-266 bp 1.0 84.4
trnH-GUG-psbA_p5 CGCATGGTGGATTCACAATC CCTCTAGACCTAGCTGCTGT 50.0 55.0 57.5 57.9 276-280 bp 1.0 81.4