Hotspot

rps18

LSC · 64809-65321 · 513 bp

Back to Anthoxanthum

Pi 0.0195

MarkerSeek score 67.70

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 513
variable_sites: 10
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGACTACTTCTTCTGGATCCGA	GGTAGGAATCAACGAGCTGG	45.5	55.0	57.8	58.1	672-699 bp	1.0	65.9
rps18_p2	GACTACTTCTTCTGGATCCGA	GGTAGGAATCAACGAGCTGG	47.6	55.0	56.3	58.1	671-698 bp	1.0	62.2
rps18_p3	AGACTACTTCTTCTGGATCCG	GGTAGGAATCAACGAGCTGG	47.6	55.0	56.3	58.1	672-699 bp	1.0	62.1
rps18_p4	AGACTACTTCTTCTGGATCCGA	TGGTAGGAATCAACGAGCTG	45.5	50.0	57.8	57.3	673-700 bp	1.0	61.0
rps18_p5	AGACTACTTCTTCTGGATCCGA	GGTCTGGCCCTTTCGAATAA	45.5	50.0	57.8	57.6	630-633 bp	1.0	60.4