Hotspot

atpF

LSC · 32544-33944 · 1401 bp

Back to Anthoxanthum

Pi 0.00433

MarkerSeek score 62.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1401
variable_sites: 6
pi: 0.00433
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGGCGAGCAAAGTAAGTGAT	TTCGGAAGCAAGGGGAAAAA	50.0	45.0	58.3	57.9	1523-1538 bp	1.0	78.4
atpF_p2	GGGCGAGCAAAGTAAGTGAT	TTTCGGAAGCAAGGGGAAAA	50.0	45.0	58.3	57.9	1524-1539 bp	1.0	78.4
atpF_p3	GGGCGAGCAAAGTAAGTGAT	TTTTCGGAAGCAAGGGGAAA	50.0	45.0	58.3	57.9	1525-1540 bp	1.0	78.4
atpF_p4	GGGCGAGCAAAGTAAGTGAT	TTTTTCGGAAGCAAGGGGAA	50.0	45.0	58.3	57.9	1526-1541 bp	1.0	78.4
atpF_p5	AAAGGGCGAGCAAAGTAAGT	TTCGGAAGCAAGGGGAAAAA	45.0	45.0	57.7	57.9	1526-1541 bp	1.0	78.3