Hotspot

ndhC-trnV-UAC

LSC · 49740-50654 · 915 bp

Back to Anthoxanthum

Pi 0.00656
MarkerSeek score 62.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
915
variable_sites
6
pi
0.00656
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCCTGAAATCCAAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 58.0 57.9 1025 bp 1.0 79.7
ndhC-trnV-UAC_p2 GCCCTGAAATCCAAAATGCC CGGTTCGAATCCGTATAGCC 50.0 55.0 58.0 58.3 1026 bp 1.0 77.9
ndhC-trnV-UAC_p3 CTCTGGTCCTTCACTAACCG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.3 57.9 1054 bp 1.0 74.7
ndhC-trnV-UAC_p4 TCTGGTCCTTCACTAACCGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.7 57.9 1053 bp 1.0 74.0
ndhC-trnV-UAC_p5 TCACTAACCGGGGCTAAAAG GGTTCGAATCCGTATAGCCC 50.0 55.0 57.2 57.9 1044 bp 1.0 73.7