Hotspot

trnC-GCA-rpoB

LSC · 18238-19398 · 1161 bp

Back to Anthoxanthum

Pi 0.00618

MarkerSeek score 64.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1161
variable_sites: 7
pi: 0.00618
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1203-1230 bp	1.0	81.4
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1210-1237 bp	1.0	80.3
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1218-1245 bp	1.0	79.1
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1217-1244 bp	1.0	79.1
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	50.0	45.0	58.1	57.6	1282-1309 bp	1.0	78.6