Hotspot

trnY-GUA-trnD-GUC

LSC · 15622-15998 · 377 bp

Back to Anthoxanthum

Pi 0.0111

MarkerSeek score 64.20

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 4
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	50.0	50.0	58.0	58.5	487-502 bp	1.0	79.1
trnY-GUA-trnD-GUC_p2	AATTCTTCCTGGGTCGATGC	ACTCTAGTTCGGGACTGACG	50.0	55.0	58.0	58.5	540-555 bp	1.0	78.2
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	454-469 bp	1.0	76.8
trnY-GUA-trnD-GUC_p4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	453-468 bp	1.0	76.7
trnY-GUA-trnD-GUC_p5	GGGGACGGACTGTAAATTCG	ACTCTAGTTCGGGACTGACG	55.0	55.0	58.4	58.5	507-522 bp	1.0	75.9