Hotspot

psbA

LSC · 334-1395 · 1062 bp

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Pi 0.00282

MarkerSeek score 48.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TGCTCCTTTATTTTCAAAAACTCGT	TTGTGTACTTGGGAGTCCCT	32.0	50.0	58.2	57.9	1153 bp	1.0	40.2
psbA_p2	TGCTCCTTTATTTTCAAAAACTCG	TTGTGTACTTGGGAGTCCCT	33.3	50.0	56.7	57.9	1153 bp	1.0	40.2
psbA_p3	GCTCCTTTATTTTCAAAAACTCGT	TTGTGTACTTGGGAGTCCCT	33.3	50.0	56.7	57.9	1152 bp	1.0	40.2
psbA_p4	TGCTCCTTTATTTTCAAAAACTCGT	ACTTGGGAGTCCCTGATGAT	32.0	50.0	58.2	57.7	1147 bp	1.0	40.2
psbA_p5	TGCTCCTTTATTTTCAAAAACTCGT	GTGTACTTGGGAGTCCCTGA	32.0	55.0	58.2	58.4	1151 bp	1.0	40.2