Hotspot

atpF

LSC · 12354-12908 · 555 bp

Back to Anthoshorea

Pi 0.00721

MarkerSeek score 47.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 4
pi: 0.00721
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGTAAGCCTCACTCACTGGT	AAAGTATCGCGAGACAACCC	50.0	50.0	58.0	58.0	1660-1723 bp	1.0	81.3
atpF_p2	TACCTGTTTGCCTATCCCCA	AAAGTATCGCGAGACAACCC	50.0	50.0	58.0	58.0	1873-1936 bp	1.0	81.1
atpF_p3	AGTAAGCCTCACTCACTGGT	CCCAGGCGGATGGAAAAATA	50.0	50.0	58.0	57.9	1642-1705 bp	1.0	80.9
atpF_p4	CGGCTTTAATGGTTGCCATG	AAAGTATCGCGAGACAACCC	50.0	50.0	58.1	58.0	1366-1429 bp	1.0	80.8
atpF_p5	TACCTGTTTGCCTATCCCCA	CCCAGGCGGATGGAAAAATA	50.0	50.0	58.0	57.9	1855-1918 bp	1.0	80.6