Hotspot

rpl23-trnI-CAU

IRb · 86007-86276 · 270 bp

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Pi 0.0596
MarkerSeek score 74.60
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
IRb
length_bp
270
variable_sites
14
pi
0.0596
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 TGATTTTTCGCCAATAACCGA GAATGGTTAAAGCGCCCAAC 38.1 50.0 56.5 58.0 359-394 bp 1.0 67.1
rpl23-trnI-CAU_p2 TGATTTTTCGCCAATAACCGA CCTGTTAAGCATCCATGGCT 38.1 50.0 56.5 57.9 379-414 bp 1.0 66.5
rpl23-trnI-CAU_p3 TGATTTTTCGCCAATAACCGAA GAATGGTTAAAGCGCCCAAC 36.4 50.0 57.2 58.0 359-394 bp 1.0 64.8
rpl23-trnI-CAU_p4 TTGATTTTTCGCCAATAACCGA GAATGGTTAAAGCGCCCAAC 36.4 50.0 57.2 58.0 360-395 bp 1.0 64.7
rpl23-trnI-CAU_p5 TGATTTTTCGCCAATAACCGAA CCTGTTAAGCATCCATGGCT 36.4 50.0 57.2 57.9 379-414 bp 1.0 64.2