Hotspot

ndhC-trnV-UAC

LSC · 51781-52792 · 1012 bp

Back to Anthoshorea

Pi 0.0168

MarkerSeek score 68.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 17
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCTTTTCGAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	1155-1216 bp	1.0	79.4
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1144-1205 bp	1.0	78.1
ndhC-trnV-UAC_p3	TCGAATCGGGGCTAAAACTC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1150-1211 bp	1.0	77.2
ndhC-trnV-UAC_p4	CCTTTTCGAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.0	57.5	1154-1215 bp	1.0	76.6
ndhC-trnV-UAC_p5	TCTGGACCTTTTCGAATCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1161-1222 bp	1.0	76.6