Hotspot

trnfM-CAU-rps14

LSC · 37076-37243 · 168 bp

Back to Anthoshorea

Pi 0.0314
MarkerSeek score 70.60
Primer available yes
Label trnfM-CAU-rps14

Feature Metadata

label_name
trnfM-CAU-rps14
feature_type
igs
region
LSC
length_bp
168
variable_sites
5
pi
0.0314
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-rps14_p1 AAGGTTATGAGCCTTGCGAG TTGGGCTATCCGGGCATATA 50.0 50.0 58.0 58.0 279-288 bp 1.0 83.4
trnfM-CAU-rps14_p2 TTGCGGGAATGGGATTTGAA TTGGGCTATCCGGGCATATA 45.0 50.0 58.1 58.0 310-319 bp 1.0 82.7
trnfM-CAU-rps14_p3 AAATGTTGCGGGAATGGGAT TTGGGCTATCCGGGCATATA 45.0 50.0 58.1 58.0 315-324 bp 1.0 82.1
trnfM-CAU-rps14_p4 AATGTTGCGGGAATGGGATT TTGGGCTATCCGGGCATATA 45.0 50.0 58.1 58.0 314-323 bp 1.0 82.1
trnfM-CAU-rps14_p5 ATGTTGCGGGAATGGGATTT TTGGGCTATCCGGGCATATA 45.0 50.0 58.1 58.0 313-322 bp 1.0 82.1