Hotspot

petN-psbM

LSC · 27985-28689 · 705 bp

Back to Anthoshorea

Pi 0.0256

MarkerSeek score 66.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 17
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TGTCACTCGTAGTATGGGGA	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.2	58.3	816-821 bp	1.0	77.0
petN-psbM_p2	ACATTTTCCCTGTCACTCGT	ATTGCTACTGCGCTGTTCAT	45.0	45.0	57.1	58.3	826-831 bp	1.0	76.0
petN-psbM_p3	TGTCACTCGTAGTATGGGGA	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.2	57.6	817-822 bp	1.0	76.0
petN-psbM_p4	TGTCACTCGTAGTATGGGGA	TTGCTACTGCGCTGTTCATT	50.0	45.0	57.2	58.5	815-820 bp	1.0	75.3
petN-psbM_p5	ACATTTTCCCTGTCACTCGT	TATTGCTACTGCGCTGTTCA	45.0	45.0	57.1	57.6	827-832 bp	1.0	75.0